Since, the question is incomplete the complete question is as follows:
Choose one or more:
Probabilistic and dichotomous keys for microbial identification would not be used in situations where:
A. microscopic examination is possible.
B. the organism is uncultured.
C. species are incompletely characterized.
D. isolates are of clinical significance.
E. time is of the essence.
Answer: B. the organism is uncultured.
C. species are incompletely characterized.
E. time is of the essence.
Step-by-step explanation:
For the process of identification of microbial species probabilistic and dichotomous keys are used. For utilizing these methods for the identification of microbes which is uncultured is likely to fail the methods for identification, as several tests involving these methods will require the examination of the culture for observing various characteristics.
According to these methods one of two options are selected for identification then further the process is repeated, and the process is completed when the tip of tree is reached. The end is indicative of genus or species.
These methods are not easy to work with and the species is incompletely characterized. It is time consuming too.