Question

A flask containing 100ml of YNB broth was inoculated with approximately yeast cells. The flask was incubated at 37 degrees Celsius (100rpm). Samples were periodically collected and their absorbance (optical density-OD) was measured:

Using the given data (shown in photo) calculate the doubling time of this microorganism (FIRSTLY, you have to determine the exponential phase!)

Answer 1

The time it takes for the yeast population to double is 1 hour.

Step-by-step explanation:

Convert the OD to an approximation of the number of cells present in the broth. Do this by multiplying the OD value with 3 x 10^7.

The exponential phase of growth for the yeast cells is from

time t = 0 hours to time t = 5 hours