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You study a gene called hmr-1, which is essential for cell-cell adhesion. You find that it is expressed in specific cells, but you still do not know where the HMR-1 protein is localized inside the cell (e.g. nucleus, transmembrane, cytoplasm). In order to learn about HMR-1 protein localization, which molecular construct would you build to be later transfected into cells?

A. gtp gene (coding region, 5 and 3 UTRs) fused downstream to the hm-1 gene. The hml-1 gene will include its original regulatory sequences as well as its coding region and its S'UTR) and 3 UTRs) used downstream to the gtp gene. The gtp gene will include its original regulatory sequences as well as its
B. hmt gene (coding region, coding region and its SUTR)
C. gtp gene (coding region, 5 and 3 UTRs) fused to the gtp regulatory sequences
D. hmr-1 gene (coding region, 5' and 3' UTRs), fused to the gtp regulatory sequences

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Answer:

A. gtp gene (coding region, 5 and 3 UTRs) fused downstream to the hm-1 gene. The hml-1 gene will include its original regulatory sequences as well as its coding region and its S'UTR) and 3 UTRs) used downstream to the gtp gene. The gtp gene will include its original regulatory sequences as well as its

Step-by-step explanation:

when the fusion between GFP and the hmr-1 gene occurs downstream, the location of the latter can be monitored by means of fluorescence

User Stephan Luis
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