Question

Avidin is a protein from egg white. It has very high affinity to biotin and because of that works as a highly specific inhibitor of biotin enzymes. Which of the following conversions would be blocked by the addition of avidin to a cell homogenate?

(a) Glucose → pyruvate

(b) Pyruvate → glucose

(c) Oxaloacetate → glucose

(d) Malate → oxaloacetate

(e) Pyruvate → oxaloacetate

(f) Glyceraldehyde 3-phosphate → fructose 1,6-bisphosphate

Answer 1

B and E

Step-by-step explanation:

1. Avidin is a glycoprotein, four identical subunits are there in avidin. It is found in white part of the egg of reptiles, amphibians, and birds.

2. Avidin binds in specific manner with biotin.

3. Avidin is resistant to some of the enzymes like pancreatic enzymes.

4. The conversions of Pyruvate into glucose and and the conversion of Pyruvate into oxaloacetate would be blocked if the avidin is added to a cell homogenate.