Question

According to the Lab Safety Sheet provided for this week, which of the following is a potential hazard you will face in the b-galactosidase assay lab? (Check all that apply)Select one or more:

a. Water reactive chemicals
b. Corrosive chemicals
c. Chemicals causing respiratory tract, eye and skin irritation
d. Potentially carcinogenic chemicals
e. Radioactive materials

Answer 1

In the b-galactosidase assay lab, potential hazards include corrosive chemicals, chemicals causing respiratory tract, eye and skin irritation, and potentially carcinogenic chemicals.

Step-by-step explanation:

In the b-galactosidase assay lab, potential hazards you may face include:

• Corrosive chemicals: These can burn your skin and eyes, causing harm.
• Chemicals causing respiratory tract, eye and skin irritation: Some chemicals used in the lab can cause irritation to your respiratory tract, eyes, and skin.
• Potentially carcinogenic chemicals: There may be chemicals in the lab that have the potential to cause cancer.

Answer 2

Chemicals causing respiratory tract, eye and skin irritation.

Potential Carcinogenic chemicals

Step-by-step explanation:

b-galactosidase is an enzyme encoded by Lac Z gene of the lac operon in E. coli. It hydrolyses b-galactosides and lactose into monosaccharides which are simple sugars

In order to carry out this assay, materials including b-mercaptoethanol and ONPG(o-nitrophenyl-b-d-galactopyranoside) are used.

b-Mercaptoethanol produces vapors which can cause respiratory tract irritation if inhaled and ONPG is a potential carcinogenic chemical.