Question

As the most junior member of a lab, you are tasked with generating cell lines that accumulate DNA damage to investigate how random mutations affect transformation of cells into cancer cells. You decide to mutate proteins in the p53 pathway. Which three proteins would you mutate

Answer 1

In p53 pathways, 3 proteins which are mutate and cause cancer that proteins are:

1.BRCA2

2. p53 in Rb genes binds with deoxyribonucleic acid that stimulates another gene to produce a protein known as p21 that interacts with cdk2. When p21 is combined with cdk2 cell will not pass through to the next stage of cell division. This is Li-Fraumeni syndrome (when p53 causes cancer from parents to offspring)

3.BRCA1

The parent that transmits mutation in one of these genes has a 50% chance of inheriting the mutation. The effects of mutations in BRCA2 and BRCA1 are seen when the person's second copy of the gene is normal.