Question

After re-folding the protein lolcat31, you perform gel filtration to complete the purification. You calculate the size of lolcat31 to be 35,000 daltons from its amino acid sequence. Instead, however, the protein elutes at 140,000 daltons. Addition of Triton X-100, a chemical used by biochemists to disrupt hydrophobic interactions, causes the protein to elute at 70,000 daltons.

a. lolcats31 contains two polypeptide chains, all of which are held together by hydrophobic forces
b. lolcats31 contains four polypeptide chains, two of which are held together by hydrophobic forces
c. lolcats31 contains two polypeptide chains, all of which are held together by disulfide bridges (-S-S-)
d. lolcats31 contains four polypeptide chains, and is entirely denatured by addition of Triton-X100
e. lolcats31 contains four polypeptide chains, all of which are held together by hydrophobic forces.

Answer 1

The answer is "Option b".

Step-by-step explanation:

Proteins act molecules consist entirely in a fixed order of one or even more fatty acids chains, defined by a nucleotide throughout the protein-coding DNA. That's why the lolcats31 consists of 4 polypeptides, two of whom are hyDrophobic factors holding together, and protein is required to form, work, and regulate the structures, tissues, and organs, and tissues.