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Start with the temperature at 25 ºC and the pH at 7. Do not vary these parameters while testing for substrate (Initial Lactose) concentration, as they are environmental controls. Substrate (Initial Lactose) concentration is in mg/dL.

Vary the substrate (Initial Lactose) concentration by 250 mg/dL, starting with 0 mg/dL.
Click the green ‘Run’ button.
After the experiment runs, click the graph between 2 and 4 minutes and move it until the time underneath the graph shows ‘Minutes: 3’ and hold it there.

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Answer:

Cooler temperatures slow the rate of lactase's function, while at extremely high temperatures -- those above 135 degrees Fahrenheit, for example -- lactase can become denatured, or lose its shape. A protein's shape is responsible for its function, so when it becomes denatured, lactase loses its ability to function.

Step-by-step explanation:

User Rob Truxal
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