Answer:
Enzyme activity depends on many factors such as Substrate concentration, enzyme cofactor, enzyme inhibitors, pH, and temperature. pH and temperature are two important factors that directly affect the activity of an enzyme as follows:
1. pH
Each enzyme has the specific optimal pH that allows it to perform its function to its fullest. The enzymes are the protein made up of the interaction of the hydrogen bond in the side chain of the protein. if there is any change from optimum pH, the enzyme activity decreases until it stops working.
2. Temperature:
Each enzyme has its own optimal temperature. Any modification or change in temperature results in the activity of an enzyme, and it also leads to the denaturation of an enzyme as it is made up of protein.