Final answer:
To make DNA in vitro using DNA polymerase I, one would need a DNA template, primers, nucleotides, and the DNA polymerase I enzyme itself, with the DNA template serving as the sequence guide, the primers providing the initiation point, and the nucleotides being the building blocks added to the growing DNA strand. The correct option is A.
Step-by-step explanation:
The necessary components to make DNA in vitro using DNA polymerase I are a DNA template, primers, nucleotides, and DNA polymerase I itself. The correct answer to this question is A) DNA template, primers, nucleotides, and DNA polymerase I. DNA polymerase I is an enzyme that synthesizes DNA molecules from deoxyribonucleotides, the building blocks of DNA.
These enzymes are essential to DNA replication and usually work in pairs to create two identical DNA strands from a single original DNA molecule. During this process, DNA polymerase I reads the existing DNA strands to create two new strands that complement each other.
The DNA template provides the sequence pattern for the new strand, while primers are short nucleic acid sequences that provide a starting point for DNA synthesis. The primers attach to the template strand and are extended by the polymerase with nucleotides, which are the building blocks of DNA.
These nucleotides are added to the primer in a sequence-specific manner to form the new DNA strand. Therefore, the primer provides the free 3'-OH end required by DNA polymerase for nucleotide addition.