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A master dilution is utilized in the titration technique for the purpose of:

A) Decreasing analytical error in the procedure.

B) Comparing multiple samples from the same patient.

C) Increasing likelihood of detecting antibody presence.

D) Comparing the reactivity of a single antibody against several red cell samples.

User Mark VY
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Final answer:

A master dilution is used in titration techniques to reduce analytical errors, providing precision in measurements when determining antibody concentrations or viral titers, and is undertaken using precise tools like pipettes.

Step-by-step explanation:

A master dilution in titration is primarily utilized for the purpose of decreasing analytical error in the procedure. During laboratory practices, such as quantitative assays and serological testing, serial dilutions are often performed to obtain accurate measurements of the concentration of a substance, like an antibody in a patient's serum. The use of dilution helps in distinguishing whether an infection is current or past by comparing antibody titers in different phases, and it allows for the comparison of reactivity across several samples.

Serial two-fold dilutions are commonly used, for instance, when measuring the titer of agglutinating antibodies or when determining the concentration of viruses using hemagglutination assays (HA). In these techniques, tools such as glass pipettes and micropipettes play a pivotal role in ensuring precision and accuracy.

Furthermore, the concept of dilution is also found in processes like neutralization reactions in titrations, where it aids in reaching an endpoint that signals the completion of the reaction. This precise approach contributes significantly to reducing the margin of error and enhancing the reliability of experimental data.

User Joe Allen
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