Final answer:
To determine the DNA fingerprint of the victim and three suspects, their DNA needs to be cut using the restriction enzyme Not1. The fragment sizes for each DNA fingerprint will depend on the number and locations of the Not1 recognition sites. To determine the guilty suspect, compare the DNA fingerprints of the suspects with the DNA fingerprint of the victim.
Step-by-step explanation:
To determine the DNA fingerprint for the victim and three suspects, we need to cut their DNA using the restriction enzyme Not1. Not1 recognizes the sequence GGGCCC and cuts at the G/C. By cutting the DNA, we can produce fragments of different sizes that can be visualized using gel electrophoresis.
The fragment sizes for each DNA fingerprint will depend on the number and locations of the Not1 recognition sites in their DNA. If we assume that the Victim has one recognition site, Suspect 1 has two recognition sites, Suspect 2 has three recognition sites, and Suspect 3 also has three recognition sites, we can estimate the fragment sizes as follows:
- Victim: One large fragment (length depends on the location of the recognition site)
- Suspect 1: Two fragments (one larger than the Victim's fragment, and one smaller)
- Suspect 2: Three fragments (one larger than Suspect 1's larger fragment, one between Suspect 1's fragments, and one smaller than Suspect 1's smaller fragment)
- Suspect 3: Three fragments (similar to Suspect 2)
To determine which suspect is most likely guilty of the murder, we would need to compare the DNA fingerprints of the suspects with the DNA fingerprint of the victim. If the DNA fingerprints of the suspects match the fragments observed in the victim's DNA fingerprint, then it would indicate a higher likelihood of guilt.