Final answer:
The correct sequence for the production of humulin using recombinant DNA technology is: Synthesis of DNA for human insulin, insertion of this gene into a plasmid, introduction of the plasmid into E. coli, culturing the bacteria, extracting the insulin, and purifying it. The correct option is o i, iv, v, ii, vi, iii. The correct answer is option 3.
Step-by-step explanation:
Correct Sequence in the Production of Humulin
The production of humulin, or synthetic human insulin, involves several steps using recombinant DNA technology. The correct sequence of these steps is (i) Synthesis of gene (DNA) for human insulin artificially, (iv) Insertion of human insulin gene into plasmid, (v) Introduction of recombinant plasmid into E. coli, (ii) Culturing recombinant E. coli in bioreactors, (vi) Extraction of recombinant gene product from E. coli, and finally (iii) Purification of humulin. Therefore, the correct option is: (iii) o i, iv, v, ii, vi, iii.
Starting with synthetically created DNA sequences specific to the A and B polypeptide chains of insulin, the genes are introduced into a bacterial plasmid. They are then propagated in E. coli cells allowing for the production of insulin. Finally, insulin is extracted and purified to yield a medication that can be used to effectively treat patients with diabetes, with significantly lower risk of adverse allergic reactions compared to animal-derived insulin.
Recombinant DNA technology has provided a means to produce vital proteins like insulin in a cost-effective and patient-friendly way. The entire process illustrates the powerful application of genetic engineering in medicine.