Final answer:
One can deduce the complementary DNA strand from a single strand using base-pairing rules. The sequence runs antiparallel with A pairing with T and G pairing with C. During replication, primase and SSBs play crucial roles in the formation of this complementary strand.
Step-by-step explanation:
Given one single DNA strand, one can deduce the sequence of the other strand, which is known as the complementary strand. DNA strands run antiparallel, meaning if one strand is oriented 5' to 3', then the complementary strand runs 3' to 5'. The two strands are held together by hydrogen bonds between specific nitrogenous base pairs: adenine (A) pairs with thymine (T), while guanine (G) pairs with cytosine (C).
For the given sequence 5'CGAGTACG3', the complementary strand would be 3'GCTCATGC5', following the base-pairing rules. During DNA replication, the enzyme primase synthesizes a short RNA primer on which DNA polymerase can add nucleotides, synthesizing the complementary strand. The single-stranded binding proteins (SSBs) stabilize the unwound strands preventing them from reannealing or forming secondary structures.