Question

What is the difference between the way in which X-ray crystallography and cryo-EM

determine molecular structure as compared with the way AFM accomplishes the same goal?
1)X-ray crystallography and cryo-EM are much more exact than AFM.
2)AFM is much more exact that X-ray crystallography and cryo-EM.
3) X-ray crystallography and cryo-EM average the structure of many individual molecules, while AFM provides an image of each individual molecule as it is oriented in the field.
4)AFM averages the structure of many individual molecules, while X-ray
crystallography and cryo-EM provide an image of each individual molecule as it is oriented in the field.
A) 1
B) 2
C) 3
D) 4
E) 1 and 3

Answer 1

X-ray crystallography and cryo-EM provide an image of individual molecules, while AFM averages structures of many molecules.

Step-by-step explanation:

The difference between X-ray crystallography and cryo-EM compared to AFM in determining molecular structure is that X-ray crystallography and cryo-EM provide an image of each individual molecule as it is oriented in the field, while AFM averages the structure of many individual molecules.

X-ray crystallography measures the diffraction of X-rays by the crystal to determine the size and arrangement of atoms in the crystal.

Cryo-EM, on the other hand, uses an electron microscope to capture images of individual molecules. AFM, or atomic force microscopy, uses a tiny probe to scan the surface of a sample, providing information about height and surface properties.

Trends within the periodic table, such as ionization energy increasing across a row and decreasing down a column, aid in pinpointing the element based on the spectrum.