Final answer:
Two factors that need to be controlled in generating data on H₂O₂ concentration and catalase activity are temperature and pH.
Step-by-step explanation:
To generate data on H₂O₂ concentration and catalase activity, two factors that need to be controlled are temperature and pH.
Temperature: The activity of enzymes, such as catalase, is highly dependent on temperature. As the temperature increases, the enzymatic activity also increases, up to a certain point known as the optimum temperature. Beyond the optimum temperature, the activity starts to decline.
Therefore, in order to obtain reliable data, the experiment should be conducted at a constant temperature, preferably at the enzyme's optimum temperature.
pH: Enzymes also have an optimal pH range within which they function most efficiently. pH affects the ionization state of amino acids in the enzyme's active site, which in turn affects its catalytic activity. To control this factor, the experiment should be performed at a specific pH level, usually the enzyme's optimal pH.