Question

When creating a separation method in gas chromatography, calibration with compounds mixture or for each separate component?

Answer 1

In gas chromatography, calibration can be done with a compound mixture or for each separate component, depending on the specific situation.

Step-by-step explanation:

In gas chromatography, the calibration can be done with a compound mixture or for each separate component, depending on the specific situation.

When using a compound mixture for calibration, a mixture containing known amounts of different compounds is used to establish reference peaks and retention times. This allows for the identification and quantification of unknown components in a sample based on their retention times relative to the reference peaks.

Alternatively, calibration can be performed for each separate component by analyzing individual standards of each compound of interest. This approach is useful when the sample contains a specific mixture that requires the separation of its components.