Final answer:
Double-stranded DNA will separate into two strands when exposed to high temperature.
Step-by-step explanation:
Double-stranded DNA will separate into two strands when exposed to high temperature. When DNA is subjected to temperatures above 80 to 90 degrees Celsius, it gets denatured and the two strands separate completely from each other. This process is called DNA denaturation. The ability to artificially manipulate DNA in this way is the basis for several important techniques in biotechnology.