Question

"E. coli has two types of priming reaction, which occur at"

Answer 1

E. coli has two types of priming reaction, occurring at the N8 and N9 positions. The wild-type enzyme and the D204N mutant enzyme have different ribosylation products when reacting with different substrates. Mutated enzymes have lower ribosylation rates.

Step-by-step explanation:

E. coli has two types of priming reaction, which occur at the N8 and N9 positions. When reacting with the substrate 8-azaguanine, the wild-type enzyme primarily produces N9-riboside, while the D204N mutant enzyme produces mainly N9-nucleoside. However, when reacting with the substrate 8-azaDaPur, the N8-riboside becomes the dominant product for the wild-type enzyme. The mutant enzyme in this case still produces N9-nucleoside. The mutated enzymes generally have lower ribosylation rates compared to the wild-type enzyme.