Question

Restriction enzyme digestion would NOT be used for which of the following techniques?

A Restriction fragment length polymorphism (RFLP)
B Cloning a PCR fragment
C Southern blot
D Northern blot
E Construction of a BAC library

Answer 1

Restriction enzyme digestion is not typically used for Northern blot which focuses on RNA analysis rather than DNA, where restriction enzymes find their application.

Step-by-step explanation:

Restriction enzymes cleave specific DNA sequences and are fundamental in various biotechnological applications. Out of the techniques listed, restriction enzyme digestion would NOT be typically used for Northern blot. This technique involves the study of RNA, not DNA, and although RNA may be subsequently reverse transcribed into cDNA, restriction enzymes are not part of the primary Northern blot methodology. In contrast, RFLP, cloning PCR fragments, Southern blots, and construction of BAC libraries all involve the use of restriction enzymes to digest DNA for analysis or manipulation.