Final answer:
False. The organic layer is not always the top layer when organic and aqueous phases separate; it depends on the relative densities of the liquids involved.
Step-by-step explanation:
The statement that the organic layer is always the top layer when organic and aqueous phases separate into layers is generally false. The density of the organic solvent relative to water determines its position as the top or bottom layer. In many cases, organic solvents are less dense than water and will form the top layer, such as when oil and water are mixed. However, there are also organic solvents that are heavier than water and will form the bottom layer when mixed with an aqueous solution.