Final answer:
The Mission San Luis de Talimali had a negative effect on the Native American tribes of Florida, resulting in a loss of population and culture.
Step-by-step explanation:
The effect of the Mission San Luis de Talimali on the Native American tribes of Florida was a loss of population and culture.
The Spanish colonization led to the displacement of the local Timucua Natives and the introduction of diseases that caused a significant decline in their population. By 1700, only a small number of Timucua remained. Additionally, the destruction of Indian missions by British forces further weakened the Native American tribes and their cultural practices.