Question

Sort the four bacterial classes into different categories based on the medium in which they will grow.

Given:
A) Bacteria with no plasmid (transformation did not occur)
B) Transformed bacteria containing a nonrecombinant plasmid
C) Transformed bacteria containing a recombinant plasmid without the vgp gene
D) Transformed bacteria containing a recombinant plasmid with the vgp gene
Based on the categories:
~Will grow only in medium without ampicillin
~Will grow only in medium containing ampicillin
~Will grow in both media

Answer 1

The four bacterial classes can be sorted into different categories based on the medium in which they will grow: without ampicillin, with ampicillin, or both. Each category corresponds to a specific type of plasmid and its associated traits.

Step-by-step explanation:

Based on the categories given, we can sort the four bacterial classes as follows:

1. Bacteria with no plasmid (transformation did not occur) will grow only in medium without ampicillin.
2. Transformed bacteria containing a nonrecombinant plasmid will grow in both media (with and without ampicillin).
3. Transformed bacteria containing a recombinant plasmid without the vgp gene will grow only in medium with ampicillin.
4. Transformed bacteria containing a recombinant plasmid with the vgp gene will grow in both media (with and without ampicillin).

For example, bacteria with no plasmid will not survive in the presence of ampicillin, while bacteria with a nonrecombinant plasmid will grow in both media since they have the ampicillin resistance gene. On the other hand, transformed bacteria with a recombinant plasmid but without the vgp gene will only grow in the presence of ampicillin, while bacteria with a recombinant plasmid containing the vgp gene will grow in both media.