Final answer:
The largest nationally representative and comprehensive study on sexual behaviors is the National Survey of Sexual Health and Behavior, published in 2010 by the Centre for Sexual Health Promotion at Indiana University.
Step-by-step explanation:
The largest nationally representative and comprehensive study conducted on sexual behaviors is the National Survey of Sexual Health and Behavior (NSSHB). Initiated by researchers at the Centre for Sexual Health Promotion at Indiana University, this landmark survey was published in the Journal of Sexual Medicine in 2010. The NSSHB provided rich data on a range of sexual experiences and practices among the US population. Its findings paint a broad picture of sexual health and behavior, contributing significantly to our understanding of human sexuality. This research has been particularly important in dispelling myths and challenging outdated perceptions related to gender and sexuality.