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The binding of TBP to a polymerase II promoter:

a. causes a dramatic distortion in the conformation of DNA
b. results in the insertion into the minor groove of the double helix
c. results in the insertion into the major groove of the double helix
d. causes a bend of greater than 80 degrees at the site of DNA protein interaction
e. a,b, and d

User Brunns
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1 Answer

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Final answer:

The correct answer is option e, which combines the effects of TBP binding to a polymerase II promoter, causing both a dramatic distortion in the DNA conformation and a bend of greater than 80 degrees, crucial for initiating transcription.

Step-by-step explanation:

The binding of the TATA-binding protein (TBP) to a polymerase II promoter is a crucial step in the initiation of transcription in eukaryotic cells. When TBP binds to the DNA at the TATA box within the promoter region, it causes a dramatic conformational change. This distortion includes a significant bending of the DNA, which is essential for the recruitment of additional transcription factors and the RNA polymerase II itself to form the pre-initiation complex.

Option a, which states that TBP binding causes a dramatic distortion in the conformation of DNA, is correct. This conformational change is part of the process that facilitates the unwinding of DNA and the subsequent transcription initiation. Options b and c refer to the insertion into the minor or major groove of the double helix which are not directly related to TBP binding. Option d, which suggests TBP causes a bend of greater than 80 degrees at the site of DNA-protein interaction, is also correct, as this bend is crucial to open up the double helix for transcription initiation. Considering the combined effect of options a and d, the correct answer to the question is e, both a, b, and d.

User OpenFile
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