Question

Which statement is FALSE?

a) DNA (-) strand synthesis requires transfer of the primer to new template
b) Reverse transcriptase synthesizes DNA (-) strand.
c) RNase H degrades the 5end of the RNA (+), thus leaving a 3-protruding end of the strong stop DNA (-), which then moves to the second template
d) Glutamine tRNA pairs its anticodon loop with the RNA (+) strand and primes synthesis of the strong stop DNA (-).

Answer 1

The false statement is that glutamine tRNA pairs with the RNA strand to prime DNA synthesis. In reality, a specific RNA primer is used by reverse transcriptase to synthesize the DNA strand, not glutamine tRNA.

Step-by-step explanation:

The statement that is FALSE is: "Glutamine tRNA pairs its anticodon loop with the RNA (+) strand and primes synthesis of the strong stop DNA (-)." In the context of reverse transcription, which is the process of synthesizing DNA from an RNA template, it is not glutamine tRNA but rather a different species of RNA, often an RNA primer, that is used to initiate the synthesis of DNA. Specifically, the viral RNA-dependent DNA polymerase known as reverse transcriptase synthesizes DNA by using this primer. Glutamine tRNA has no established role in priming DNA synthesis in this process. Another key point is that RNase H degrades the RNA after the first strand of DNA has been synthesized, not the 5' end of RNA, and it typically leaves a 3' overhang, which can then initiate second-strand DNA synthesis.