Final answer:
qPCR, also known as real-time PCR, is a technique that allows for quantitative measurements of DNA amplification in real-time using fluorescence. Traditional PCR, on the other hand, is not quantitative and is used for DNA amplification without measurement.
Step-by-step explanation:
Real-time PCR (quantitative PCR, qPCR) and traditional PCR are similar techniques used in molecular biology. However, there are some key differences between the two. qPCR, also known as real-time PCR, allows for the monitoring of DNA amplification in real-time using fluorescence. This means that qPCR can quantitatively measure the amount of DNA present in a sample.
In contrast, traditional PCR is not quantitative and does not measure the precise amount of DNA present. Instead, it is used to amplify a specific DNA sequence. Traditional PCR involves a series of repeated heating and cooling cycles to amplify the target DNA, whereas qPCR measures the increase in double-stranded DNA in real-time.
So, while both qPCR and real-time PCR involve the use of fluorescence to monitor DNA amplification, qPCR is specifically used for quantitative measurements, whereas traditional PCR is used for DNA amplification without quantification.