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_____Bring the image into sharper focus by turning to either direction the fine adjustment knob._____To transfer the microscope from the shelf to the table, carry it with two hands. Hold the arm with one hand and place the other hand under the base for support. ___Raise the body tube by turning the coarse adjustment knob. _____Slowly turn the coarse adjustment knob to move the objective lens up until the image of the object comes into clear focus. _____Place the microscope on the table with the arm pointing towards the user. _____Check the lighting of the working area and make sure that have enough light to view the specimen._____Turn the coarse adjustment knob carefully to lower the body tube as near as it will go without touching the glass slide. Look at the stage side by side. _____Set the LPO in line with the eyepiece or ocular by turning the revolving nosepiece. As you revolve the nosepiece you hear "click" sound indicating that you set it in position.

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Final answer:

It is best to adjust the focus using the coarse focusing knob before using the fine focusing knob because the coarse focusing knob allows for large-scale movements, while the fine focusing knob allows for small-scale movements.

Step-by-step explanation:

It is best to adjust the focus using the coarse focusing knob before using the fine focusing knob because the coarse focusing knob allows for large-scale movements, while the fine focusing knob allows for small-scale movements. By using the coarse focusing knob first, you can quickly bring the image into rough focus, and then use the fine focusing knob to make small adjustments and bring the image into sharp focus.

Example: If you are trying to focus on a specimen under high magnification, using the fine focusing knob directly might cause you to overshoot the focus point due to its sensitivity, which would make it difficult to bring the image into clear focus. However, by using the coarse focusing knob first to get the image close to the focus point, you can then use the fine focusing knob to make precise adjustments and achieve a clear and sharp image.

User Yash Dayal
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