The main impact of the Anti-Federalists on the adoption of the US Constitution was their concern for preserving liberty led to the inclusion of the Bill of Rights in the ratified form of the Constitution.
Anti Federalists were against strong government, and wanted a Bill of Rights as oppose to the Federalists.
The Bill of Rights amendments introduced to the Constitution particular guarantees of personal freedoms and rights, specific limitations on the government's power in judicial and other proceedings, and clear declarations that all powers not specifically granted to the U.S. Congress by the Constitution are reserved for the states or the people.