Question

The graph above shows the progress of an enzyme-catalyzed chemical reaction. Based on the graph, this enzyme

A. works optimally at 5°C
B. works optimally at 33°C
C. begins to denature above 25°C
D. begins to denature below 20°C

Answer 1

The right answer is C. begins to denature above 25°C

In biochemistry, denaturation is the process by which a biological macromolecule, nucleic acid or protein loses its normal three-dimensional conformation: the proteins lose their folding and unfold, the paired strands of the nucleic acid duplexes separate. This three-dimensional conformation is most often necessary so that the biological macromolecules can fulfill their function and thus the inactive denaturation in general these biological macromolecules.

Denaturation can be done by:

*Temperature: The increase in temperature causes thermal agitation of the atoms of the molecule. This causes a break in intermolecular interactions.

*PH change

*Radiation

*Chemical agents: chaotropic agents, bases, acids, reducing agents, detergents, heavy metal ions.