Question

In the sanger (dideoxy) method for dna sequencing, a small amount of a dideoxynucle- otide triphosphate—say, ddctp—is added to the sequencing reaction along with a larger amount of the corresponding dctp. what result would be observed if the dctp were omitted?

Answer 1

When the first G residue is encounteredin the template, ddCTP is added and polymerization halts. Only one band will appear in thesequencing gel.

Hope this helps !

Photon