Final answer:
When a DNA nucleotide containing guanine is accidentally substituted for a DNA nucleotide containing thymine during replication, the DNA will pair guanine with cytosine instead of thymine.
Step-by-step explanation:
When a DNA nucleotide containing guanine is accidentally substituted for a DNA nucleotide containing thymine during replication, the result is a point mutation. This means that a single base pair in the DNA sequence is changed. In this case, the substitution of guanine for thymine will cause a change in the complementary base pairing. Adenine normally pairs with thymine, so with the substitution, the DNA will pair guanine with cytosine instead of thymine.