Final answer:
Annie's experiment to study the effect of glucose on bacterial colonies starts with formulating a hypothesis, like suggesting glucose accelerates bacterial growth. Observations are recorded after growth, which could show different colony sizes or morphologies, dependent on the presence of glucose as the independent variable. These steps are vital in scientific inquiry, including hypothesis formulation, observation, and testing.
Step-by-step explanation:
Annie's objective to study the effect of glucose on bacterial colonies requires the formation of a hypothesis after making some initial observations. A hypothesis could be that glucose in the environment accelerates bacterial colony growth. Once the colonies have grown, with and without glucose, observations can be recorded. For example, differences in colony size or morphology would be such observations.
Different media can affect the growth characteristics of bacteria, which leads to varying colony morphologies. Glucose may provide the necessary nutrients for more prosperous bacterial growth compared to a medium that lacks glucose such as trypticase soy agar.
Comparisons between these bacterial colonies highlight the independent variable (presence of glucose) and the dependent variable (bacterial growth). This emphasizes the critical steps in scientific inquiry: formulate a hypothesis, observe, and test. One could infer, for instance, if bacteria exhibit better growth in the presence of glucose, that glucose is a favorable nutrient for that bacteria, emphasizing the importance of glycolysis in the growth of certain bacteria.