Final answer:
In an isolated buffalo population with no incoming new individuals, new alleles primarily appear due to mutations in the reproductive cells, leading to potential genetic variations in the offspring.
Step-by-step explanation:
The isolated population of buffalos is primarily responsible for the appearance of new alleles due to mutations in cells that will become sperm or eggs. Mutations introduce new genetic variations into the gene pool, which then may be passed onto offspring. Other mechanisms like genetic drift, natural selection, and gene flow can also impact allele frequencies, but in the context of this scenario, where no new buffalos can enter the territory, mutations are the key source of new alleles.
The appearance of new alleles in a population of buffalos that is isolated and does not receive any new buffalos into their territory is primarily responsible for through mutations in cells that will become sperm or eggs. Mutations are random changes in DNA that can produce new alleles. These mutations can lead to the creation of new genetic variations in the population.