Answer:
The correct answer is D. The conclusion might not be valid since the investigation was carried out only once.
Step-by-step explanation:
In a scientific experiment, a valid conclusion can be made by performing the experiment several times with the same experimental procedure because a single time experiment can have many defects or experimental errors.
Therefore to reduce experimental errors in microbiology and to make a valid conclusion the same experiment is performed several times. So in this question, the experiment is performed only once so the chance of experimental errors is high and the conclusion made on this experiment could be wrong.
So the statement best describes the validity of this conclusion is D.