The correct answer is A) it pushed American business interests out of Manchuria.
Japan responded to gaining control over Manchuria in that it pushed American business interests out of Manchuria.
In September 1931, Japanese troops invaded Manchuria over the excuse that China had sabotaged the railway. Japan had total control of Manchuria in February 1932 and the answer of China was to ask for the intervention of the League of Nations. The Assembly of the League of Nation agreed to demand Japan to free Manchuria. Japan did not accept the resolution of the League of Nations and attacked China and the US pushed American business interests out of Manchuria.