Question

Arrange the following statements in chronological order.

(a) The Ti plasmid is isolated and prepared for the insertion of foreign DNA.
(b)The Ti plasmid is isolated and prepared for the insertion of foreign DNA.
(c)The gene for Bt toxin is isolated from the bacterium and inserted into the plasmid.
(d) The gene for Bt toxin is isolated from the bacterium and inserted into the plasmid.
(e) A single plant cell is chosen to produce a plant that now produces Bt toxin.
(f) A single plant cell is chosen to produce a plant that now produces Bt toxin.
(g)The plasmid is taken up by a bacterium.The plasmid is taken up by a bacterium.
(h)The bacterium is used to introduce the Bt toxin gene into the chromosome of plant cells.

Answer 1

The chronological order, according to the way bacterias reproduce in other organism, is:

• The Ti plasmid is isolated and prepared for the insertion of foreign DNA.

• The gene for Bt toxin is isolated from the bacterium and inserted into the plasmid
• The bacterium is used to introduce the Bt toxin gene into the chromosome of plant cells.
• A single plant cell is chosen to produce a plant that now produces Bt toxin.