Question

Which protein will migrate through a SDS-PAGE gel the fastest?

a. a positively charged protein
b. a globular protein
c. a protein with a low net charge
d. an elongated protein

Answer 1

Answer: The globular protein will move fastest

Step-by-step explanation:

The structure of the proteins depicts the time it will take to move in a SDS-PAGE. If all the conditions are kept constant in this technique then the globular proteins will move fastest.

The structure of the globular protein is complex and it becomes compact and due to this reason it moves faster.

The elongated protein is large and due to this reason it moves slower as compared to globular proteins. There is no effect of charge on the movement of proteins because the proteins are made negative using SDS.

Answer 2

Answer: (D) An elongated protein

Step-by-step explanation:

The elongated is the type of protein that migrated through the SDS-PAGE gel more fastest. The sample of the protein ar firstly boil with the beta mercaptoethanol. Then this type of boiling treatment basically break the disulphide bond that are present to run on the SDS- PAGE.

The each protein that are present gets denatured into the structure of rod shape with the SDS negative charge molecules. The elongated structure are more feasible for travelling in the gel and its velocity migration are higher. Therefore, Option (D) is correct.