Question

A student labeled 5 tubes 0, 1, 2, 3, 4, 5. Tube 0 contained undiluted protein lysate. The student used the tube 0 lysate to prepared 10-fold serial dilutions in the tubes labeled 1-5. What is the dilution of each of the tubes?

Answer 1

Tube 1: 10⁻¹, tube 2: 10⁻², tube 3: 10⁻³, tube 4: 10⁻⁴, tube 5: 10⁻⁵.

Step-by-step explanation:

Serial dilutions are dilutions that the concentration decreases by the same quantity in each successive step. It means that the undiluted will be used for the first step, then the first will be used for the second, and successively. So, for a 10-fold, the concentration must decrease 1/10 in each step, it means that the dilution will be 1/10 in the first one (because it's 1 in tube 0).

In tube 1, the dilution is 1/10 = 0.1 = 10⁻¹;

In tube 2, the dilution will decrease more 1/10, so it will be 1/100x10 = 1/100 = 0.01 = 10⁻²;

In tube 3, it will be 1/1000x1/10 = 1/1000 = 0.001 = 10⁻³

In tube 4, it will be 10⁻⁴, and

In tube 5, it will be 10⁻⁵.