Question

What role does dna ligase perform in a dna cloning experiment? how does the action of dna ligase differ from the function of restriction enzymes?

Answer 1

In a DNA cloning experiment, DNA ligase is used to generate the covalent bonds of the phosphodiester backbone to yield an intact double-stranded DNA molecule. Restriction enzymes, on the other hand, break such bonds.

Step-by-step explanation: