Answer:
The answer is C. Settlers were hopeful but had many fears.
Step-by-step explanation:
The line "But their spirits rose when they entered Chesapeake Bay. Landing parties were delighted with the 'fair meddowes ... full of flowers of divers kinds and colors,'" shows they were hopefull and content during the voyage. However, The line "But when they entered the mouth of a broad river, which they called the James in honor of their King, and made their way up into the country, new doubts must have assailed them." and "They knew that savages lived in the dense forests which lined both banks; might not strange wild beasts live there also? Might there not be fatal diseases unknown in Europe? " shows that they were scared.
Hope this helps :)