Question

For the Hill Reaction, you used DCIP dye at a concentration of 1x10-4 M. Our lab technicians have stock solution at a concentration of 100mM. Explain how you would make 100mL of the DCIP for you to use in a Hill Reaction lab.

Answer 1

by diluting the DCIP dye stock solution 1000 times. It can be done by diluting 0.1 ml of the DCIP dye stock solution with 99.9 ml of distilled water

Step-by-step explanation:

1 millimolar (mM) = 0.001 M

1.0 × 10⁻⁴ Molar (M) = 0.0001 M = 0.1 mM

Stock solution = 100 mM = 0.1 M

100 mM DCIP dye solution diluited 1000 times >> 0.1 mM = 1 x 10⁻⁴ M