Hello. You did not say what is the experiment to which this question refers, which makes it impossible for it to be answered accurately.
However, advice that should be given to anyone who should start an experiment is: establish a control treatment, provide all treatments with the same variables and the same resources, write down everything that happened in the experiment from implementation to data collection and results, make measurements and analyzes on the same date in all treatments and keep the steaks in the same environment.