Question

How can the expression of a single gene be quickly, efficiently, and specifically shut down at the transcriptional, posttranscriptional, and posttranslational stages through coordinated expression of a transcriptional repressor, an miRNA, and a ubiquitin ligase?

Answer 1

Ubiquitin ligase is a protein enzyme recruits Ubiquitin-conjugating enzyme that come with the ubiquitin and catalyzes the transfer of ubiquitin to the protein substrate of interest and destructed by the proteosome.

miRNA are small RNA molecules with no coding behavior that binds with the mRNA with the help of base pairing of complementary codes. It helps in mRNA degradation and cutting of poly-A tail of mRNA type of gene expressing.

A gene-specific transcriptional repressor helps in preventing the transcription process of the mRNA by inhibiting the binding of the RNA polymerase to the promoter.