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What is the restriction site recognized by EcoRI?

User Medi
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2 Answers

3 votes

Answer:

In biology it is used as a restriction enzyme. EcoRI creates 4 nucleotide sticky ends with 5' end overhangs of AATT. The nucleic acid recognition sequence where the enzyme cuts is G/AATTC, which has a palindromic, complementary sequence of CTTAA/G.

Step-by-step explanation:

Hopefully that helps, I had previously learned about this stuff too, super tough stuff!

User T D
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5 votes

Answer:

G|AATTC

CTTAA|G

Step-by-step explanation:

EcoRI is a bacterial restriction enzyme that cuts DNA into fragments at defined sites. These specific sites are denoted by a specific sequence:

G|AATTC

CTTAA|G

The | symbol shows the site of the cut.

These enzymes are used to create cuts in DNA to enable cloning in genetic engineering.

User Znkr
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