Question

Digest the sequences using Haelll. Person 1 GGCCTCGGCCTAGAACGGCCTAGCCG CCGGAGCCGGATCTTGCCGGATCGGC Person 2 CTGAGGCCTAAGCGATTCCCGGATATA GACTCCGGATTCGCTAAGGGCCTATAT

Answer 1

Nucleotide fragments of the sequence 1:

1. CCTAGCCGCCGGAGCCGGATCTTGCCGGATCGGC (base 19 to base 52)

2. CCTAGAACGG (base 9 to base 18)

3. CCTCGG (base 3 to base 8)

4. GG (base 1 to base 2)

Nucleotide fragments of the sequence 2:

1. CCTAAGCGATTCCCGGATATAGACTCCGGATTCGCTAAGGG (base 7 to base 47)

2. CCTATAT (base 48 to base 54)

3. CTGAGG (base 1 to base 6)

Step-by-step explanation:

HaeIII is a restriction enzyme isolated from Haemophilus aegyptius bacteria, which is widely used in molecular biology laboratories. This enzyme is an endonuclease, i.e., it cleaves phosphodiester bonds within a polynucleotide chain. HaeIII is a restriction enzyme that cleaves DNA at the recognition sequence 5′-GG/CC-3'.