Question

If you wished to obtain a pure culture of bacteria that could degrade benzene and use it as a carbon and energy source, how would you proceed

Answer 1

I will use enrichment culture

Step-by-step explanation:

Enrichment culture media involve the use of growth nutrients to support the growth of different organisms. These nutrients promotes organism growth.

Therefore, I will use the medium with benzene and benzene will serve as my only source of carbon. Amino acids and sugars will be exempted because these medium will only contain minerals.

Once it has pass through incubation for a period of time, the culture is then placed on a solid medium using benzene as carbon source. It is advisable the whole process should be carried out using glass vessels and done in a closed environment because benzene is a volatile gas and can dissolve or react will plastic vessels.