Pasteur-Disproved spontaneous generation using S-necked flasks that were open to the air but did not let microorganisms in. Broth boiled in the flasks did not become contaminated. The organisms could have come from the air and settled into the broth. To disprove the theory of spontaneous generation, Louis Pasteur devised a way to the flask that allowed oxygen in but prevented dust from entering. Through this experiment, Pasteur demonstrated that life arises from existing life.
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