Question

Using established recombinant DNA technology, you insert a gene from a human liver cell into a bacterium. The bacterium then expresses a protein corresponding to the inserted DNA. To your dismay, you discover that the protein produced is useless and is found to contain many more amino acids than does the protein made by the eukaryotic cell.

Assuming there is no mutation in the human gene, explain why this happened.
a. bacteria do not use splicing to edit RNA after its transcription.
b. rRNAs in bacterial cells contain more nucleotides than in eukaryotic cells.
c. bacterial RNA contains less stop codons.
d. amino acids in bacterial cells are specified by double codes.

Answer 1

The fact that the amino acid chain is consistently having more amino acids than what is actually present can be attested to the fact of how gene expressions can be observed in the prokaryotic cell compared to the eukaryotic one.