166k views
0 votes
Explain the process of DNA isolation.

User Amol Patil
by
7.9k points

1 Answer

4 votes

Answer:

Lysis, precipitation and purification

Step-by-step explanation:

DNA isolation is a molecular biology technique based on the extraction of DNA and subsequent purification to remove inhibitors that may hamper the process of amplification. DNA isolation consists of three steps:

1- Lysis: it consists of the mechanical disruption of the cell, where the nucleus is broken to release DNA. During this process, it is required to use enzymes and detergents such as Proteinase K

2- Precipitation: during this process, the DNA is precipitated by using alcohol and salt.

3- Purification: this process removes the impurities from DNA. Extraction is carried out by washing DNA with ethanol and water.

User Cybernetic
by
9.3k points

No related questions found

Welcome to QAmmunity.org, where you can ask questions and receive answers from other members of our community.